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Ninty four strains of lysine producing micro-organisms in culture broth during fermentation have been isolated from soil and other sources. From the comparison of the amounts of lysine produced, 6 strains have been selected as the potentially useful strains, and identified tentatively as Micrococcus sp. (S-16-4), Corynebactcrium sp. (S-27-12, S-281-3, CBY-4) and Brevibacterium sp. (M-6-71, F-629-2), respectively. From the further studies with Corynebacterium sp., S-27-12, its maximum yield was found to be 4㎎ lysine/㎖ of synthetic medium, consist of glucose(7.5%), urea(0.6%), KH₂PO₄(0.2%), Na₂HPO₄(0.05%), MgSO₄·7H₂O(0.03%), MnSO₄·4H₂O(0.001%) and FeSO₄·7H₂O(0.0005%) at pH 7.2 and 30℃ after 4 days.
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